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Biological Processes

Cell Culture, Cytotoxic Assays, Morphological examination & Data Analysis

Vision: All biological assays for application of nanomaterials in medicinal field

 

Mission: Performing cytotoxicity, biological assays , morphological examination and other serological and fluorescent assays.

 

Services:

 

1- Cytotoxicity Assays (ex, MTT, SRB)

2- Cell Bank (different cells are available, price is subject to type of cell)

3- Morphological Examination using the Inverted Fluorescence Microscope

4- Conducting serological  and fluorescent Assays upon demand (Ex.; apoptosis mechanisms, proliferation assays, etc.)

5- Data Analysis using Multifunctional Microplate reader

Accepted Samples criteria

1) Minimum Volume: 1 ml 

2) Concentration: 1mg/ ml

3) Nano fibers or nanosheets: Disc- shaped to fit the microplate format

4) Stored and packaged properly to obtain accurate results

( Inverted Fluorescence Microscope )

1)Microscope

 ZEISS Axio Observer 5

 

2) Objectives

 

10 X, 20 X, 50 X, 100 X

 

3) Illumination Modules

 

Transmitted & Reflected Light (HXP 120 V)

 

4) Camera

Large field of view with high resolution of 12 Megapixels

 

5) Software: 

ZEN lite

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( ZEISS IFM Snap Shots )

( Microplate Reader )

Multimode Microplate ELISA Reader;

Vision:

Quantitative detection of chemical, physical or biological reactions that occur during an experiment, to determine anti-cancerous, antiviral activities, associated with interaction of nanomaterials with the tested host and evaluated the interplaying pathway mechanisms.

 

Mission:

Covering many applications through different detection modes. Minimization of operational time & money to allow researchers to dedicate more time to research & data analysis.

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( Multimode Microplate ELISA Reader Detection Modes )

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( Multimode Microplate ELISA Reader Microplate Format )

Microplate Format:

6-to-1536-well plates.

( Multimode Microplate ELISA Reader Facilities )


Reagent injector, Atmospheric control unit, incubation, shaking

Reagent Injector :

Simultaneous injection & measurement to detect signals

before, during & after reagent injection.

Atmospheric Control Unit :

Can control  O2% & CO2% within the microplate chamber.

It regulates both gases from 0.1- 20%.

Incubation:

Temperature controlled from 10°C up to 65°C.

Shaking:

Complete mixing of reagents with the tested host

Microplate Washer:

Fast, reliable, and optimized microplate washing

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