Biological Processes
Cell Culture, Cytotoxic Assays, Morphological examination & Data Analysis
Vision: All biological assays for application of nanomaterials in medicinal field
Mission: Performing cytotoxicity, biological assays , morphological examination and other serological and fluorescent assays.
Services:
1- Cytotoxicity Assays (ex, MTT, SRB)
2- Cell Bank (different cells are available, price is subject to type of cell)
3- Morphological Examination using the Inverted Fluorescence Microscope
4- Conducting serological and fluorescent Assays upon demand (Ex.; apoptosis mechanisms, proliferation assays, etc.)
5- Data Analysis using Multifunctional Microplate reader
Accepted Samples criteria
1) Minimum Volume: 1 ml
2) Concentration: 1mg/ ml
3) Nano fibers or nanosheets: Disc- shaped to fit the microplate format
4) Stored and packaged properly to obtain accurate results
BS, class II, Type II
CO2/ O2 Incubator
Microplate Reader
Centrifuge 4, 900 r.p.m
Microplate Washer
 -80° Freezer |  Liquid N2 |  Ultrasonic Cleaner |
|---|
( Inverted Fluorescence Microscope )
1)Microscope
ZEISS Axio Observer 5
2) Objectives
10 X, 20 X, 50 X, 100 X
3) Illumination Modules
Transmitted & Reflected Light (HXP 120 V)
4) Camera
Large field of view with high resolution of 12 Megapixels
5) Software:
ZEN lite
( ZEISS IFM Snap Shots )
 Convallaria Majalis Fluorescence 400x |  Histology Tissue 400x |  Untreated Liver Cancer cells 100x |
|---|---|---|
 Drug Treated Human Liver Cancer cells 100x |  Hella Cells 100x |
( Microplate Reader )
Multimode Microplate ELISA Reader;
Vision:
Quantitative detection of chemical, physical or biological reactions that occur during an experiment, to determine anti-cancerous, antiviral activities, associated with interaction of nanomaterials with the tested host and evaluated the interplaying pathway mechanisms.
Mission:
Covering many applications through different detection modes. Minimization of operational time & money to allow researchers to dedicate more time to research & data analysis.
( Multimode Microplate ELISA Reader Detection Modes )
( Multimode Microplate ELISA Reader Microplate Format )
Microplate Format:
6-to-1536-well plates.
 384-well microplate. |  96-well microplate. |  LVis Plate with 16 microspots (2 µL). |
|---|---|---|
 6-well microplate. |
( Multimode Microplate ELISA Reader Facilities )
Reagent injector, Atmospheric control unit, incubation, shaking
Reagent Injector :
Simultaneous injection & measurement to detect signals
before, during & after reagent injection.
Atmospheric Control Unit :
Can control O2% & CO2% within the microplate chamber.
It regulates both gases from 0.1- 20%.
Incubation:
Temperature controlled from 10°C up to 65°C.
Shaking:
Complete mixing of reagents with the tested host
Microplate Washer:
Fast, reliable, and optimized microplate washing
